Anti-NFKBIB antibody produced in rabbit
SIGMA/AV34382 - affinity isolated antibody
Synonym: Anti-Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, β
Product Type: Chemical
Immunoblotting Cell Type: Jurkat (Cat. No. AV34382). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type Jurkat
Western Blotting Western Blot of NFKBIB in Mouse, HeLa with NFKBIB antibody at 1 μg/mL
Western Blotting Western Blot of NFKBIB in Mouse Liver with NFKBIB antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 38 kDa |
| NCBI accession no. | NP_002494   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rabbit, mouse, human, rat, guinea pig |
| storage temp. | −20°C |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q15653 |
| Application: | Rabbit Anti-NFKBIB antibody can be used for western blot applications at a concentration of 0.5 μg/ml. |
| Biochem/physiol Actions: | NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NFKBIB is a NF-κB inhibitor that forms a complex with NF-κB and traps it in the cytoplasm. Serine phosphorylation in NFKBIB proteins activates its ubiquitin-mediated degradation. This subsequently facilitates the nuclear transport and functions of NF-κB proteins. Rabbit Anti-NFKBIB antibody recognizes human, mouse, rat, canine, and bovine NFKBIB. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human NFKBIB |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: LVFGYVTEDGDTALHLAVIHQHEPF |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |