Anti-FOXH1 antibody produced in rabbit
SIGMA/AV36853 - affinity isolated antibody
Product Type: Chemical
Immunoblotting Cell Type: SP2/0 (Cat. No. AV36853). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type SP2/0
Western Blotting Western Blot of FOXH1 in Mouse NIH3T3 Whole Cell with FOXH1 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 44 kDa |
| NCBI accession no. | NP_032015   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | O88621 |
| Biochem/physiol Actions: | Foxh1 is a transcriptional activator. Foxh1 can recognizes and binds to the DNA sequence 5′-TGT[GT][GT]ATT-3′ and is required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Foxh1 forms a transcriptionally active complex containing FAST-1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Forkhead box protein H1 (FOXh1) also known as FAST-1 contains a DNA binding TGT(G/T) motif which it uses to bind SMAD2 and regulate transcription. |
| Immunogen: | Synthetic peptide directed towards the middle region of mouse FOXH1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: GPSSSSETPLWPLCSLPGPTIIEGE |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |