Anti-PTRF antibody produced in rabbit
SIGMA/AV36965 - IgG fraction of antiserum
MDL Number: MFCD02263975
Product Type: Chemical
Immunohistochemistry Anti-PTRF: Cat. No. AV36965: Immunohistochemistry of PTRF in Mouse vessel and adipocytes (skeletal muscle) tissue with PTRF antibody at 5.0 μg/mL.
Immunoblotting Cell Type: NIH/3T3 (Cat. No. AV36965). Lanes 1. Antibody dilution (concentration) 0.625 μg/mL in cell type NIH/3T3
Western Blotting Western Blot of PTRF in Mouse embryonic fibroblast with PTRF antibody at 1 μg/mL
Western Blotting Western Blot of PTRF in Mouse Lung with PTRF antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 43 kDa |
| NCBI accession no. | NP_033012   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | O54724 |
| Biochem/physiol Actions: | In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the ′Sal box′ terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Polymerase I and transcript release factor (PTRF) contains a leucine zipper motif, a nuclear localization signal, and PEST domains. It is critically involved in termination of transcription by stalling RNA poly I and releasing it from the elongating transcript. PTRF is also found localized to caveolae and regulates formation of the vesicles at the plasma membrane. |
| Immunogen: | Synthetic peptide directed towards the middle region of mouse PTRF |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: SEALPEKEGDELGEGERPEDDTAAI |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |