Anti-E2F7 antibody produced in rabbit
SIGMA/AV37583 - affinity isolated antibody
Synonym: Anti-A630014C11Rik; Anti-D10Ertd739e; Anti-E2F transcription factor 7
Product Type: Chemical
Immunohistochemistry Anti-E2F7: Cat. No. AV37583: Immunohistochemistry of E2f7 in Mouse stomach tissue with E2f7 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: SP2/0 (Cat. No. AV37583). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type SP2/0
Immunoblotting E2F7 Antibody: Cat. No. AV37583: Western Blot analysis of E2F7 in Mouse stomach cell lysates with E2F7 Antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 99 kDa |
| NCBI accession no. | NP_848724   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q6S7F2 |
| Application: | Anti-E2F7 polyclonal antibody is used to tag E2F transcription factor 7 proteins for detection and quantitation by Western blotting and in cells and tissues by immunohistochemical (IHC) techniques. It is used as a probe to study the role of E2F transcription factor 7 in the regulation of cell proliferation, especially at the level of G(1)/S gene activity during S-phase progression. |
| Biochem/physiol Actions: | E2F7 is a E2F family member. It can block the E2F-dependent activation of a subset of E2F target genes as well as mitigate cellular proliferation of mouse embryo fibroblasts |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | E2F transcription factors are key regulators of cell growth, differentiation and cell death. E2F transcription factor 7 (E2F7) which is highly expressed during mid to late S-phase binds to the promoter regions of and represses G(1)/S-regulated genes thus promoting the downswing of oscillating G(1)/S genes during S-phase progression. The previously assigned protein identifier Q8BSQ3 has been merged into Q6S7F2. Full details can be found on the UniProt database. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of mouse E2f7 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: MEVNCLTLKDLISPRQTRLDFAIED |
| Specificity: | Anti-E2F7 polyclonal antibody reacts with human, rat, canine, bovine, and mouse E2F transcription factor 7 proteins. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |