Anti-SMAD2 antibody produced in rabbit
SIGMA/AV38694 - IgG fraction of antiserum
Synonym: Anti-SMAD family member 2
Product Type: Chemical
Western Blotting JOURNAL CITATION: Hypoxic conditioned medium from human amniotic fluid-derived mesenchymal stem cells accelerates skin wound healing through TGF-β:/SMAD2 and PI3K/Akt pathways. By: Jun, E. K., Zhang, Q., et al. in Int J Mol Sci, 2014. PubMed ID: 24398984 Image collected and cropped by CiteAb from the following publication, (http://www.mdpi.com/1422-0067/15/1/605), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Cell Type: HepG2 (Cat. No. AV38694). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type HepG2
Western Blotting Western Blot of SMAD2 in transfected HEK-293 with SMAD2 antibody at 1 μg/mL
Western Blotting Western Blot of SMAD2 in Rat Liver with SMAD2 antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 51 kDa |
| NCBI accession no. | NP_001003652   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, sheep, guinea pig, rabbit, human, dog, bovine, horse, rat |
| storage temp. | −20°C |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q15796 |
| Biochem/physiol Actions: | SMAD proteins are transcription regulators that mediate multiple signal transduction pathways. SMAD2 mediates the TGF-β pathway that is involved in a wide variety of cellular processes such as cell growth, apoptosis, survival, differentiation, morphogenesis and immune responses. SMAD/TGF-β signaling accelerates skin wound healing. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human SMAD2 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: VVKRLLGWKKSAGGSGGAGGGEQNG |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |