Anti-NR2F2 (AB2) antibody produced in rabbit
SIGMA/AV39465 - IgG fraction of antiserum
Synonym: Anti-ARP1; Anti-COUP-TFII; Anti-COUPTFB; Anti-MGC117452; Anti-Nuclear receptor subfamily 2, group F, member 2; Anti-SVP40; Anti-TFCOUP2
Product Type: Chemical
Immunohistochemistry Anti-NR2F2 (ab2): Cat. No. AV39465: Immunohistochemistry of NR2F2 in Human Kidney tissue with NR2F2 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: MCF7 (Cat. No. AV39465). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type MCF7
Immunoblotting NR2F2: Cat. No. AV39465: Western blot analysis of NR2F2 in Human Fetal Kidney cell lysates with NR2F2 antibody at 1.0 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 45 kDa |
| NCBI accession no. | NP_066285   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | guinea pig, human, bovine, dog, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P24468 |
| Application: | Rabbit Anti-NR2F2 antibody is suitable for western blot (2.5 μg/ml) and IHC (4-8 μg/ml) applications. |
| Biochem/physiol Actions: | NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NR2F2 codes for a steroid hormone receptor that is involved in the differentiation of human stem cells and trophoblasts. Genetic variations in NR2F2 are associated with congenital cardiac disorders. Rabbit Anti-NR2F2 antibody recognizes human, mouse, rat, zebrafish, canine, bovine, and chicken NR2F2. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human NR2F2 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: QDEVPGSQGSQASQAPPVPGPPPGA |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |