Anti-SIRT5 (AB2) antibody produced in rabbit
SIGMA/AV40125 - IgG fraction of antiserum
Synonym: Anti-Sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Product Type: Chemical
Immunoblotting Cell Type: fetal heart (Cat. No. AV40125). Lanes 1. Antibody dilution (concentration) 5 μg/mL in cell type fetal heart
Western Blotting Western Blot of SIRT5 in Human HEK293T with SIRT5 antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 33 kDa |
| NCBI accession no. | NP_036373   |
| shipped in | wet ice |
| species reactivity | human, goat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q9NXA8 |
| Application: | Anti-SIRT5 (AB2) antibody produced in rabbit is suitable for western blot applications. |
| Biochem/physiol Actions: | SIRT5 (Sirtuin 5) is associated with metabolism and aging process. It has been reported in a study that SIRT5 influences urea cycle pathway by NAD-dependent deacetylation and subsequent activation of carbamoyl phosphate synthetase 1 (CPS1), which plays a vital role in the initial step of urea cycle for the detoxification and removal of ammonia. In non-small cell-lung cancer cells, SIRT5 contributions have also been observed for the cancer cell growth and drug resistance. Study shows that SIRT5 may possess oncogenic property. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SIRT5 (Sirtuin 5) is a mitochondrial matrix protein belonging to the class III of the sirtuin family. It is localized inside the mitochondria and within the cytosol and nucleus. The protein is widely expressed in the brain, heart, liver and kidney. It is composed of very short amino and carboxyl terminal sequences flanking region with the conserved, catalytic sirtuin center domain.The previously assigned protein identifier Q5T295 has been merged into Q9NXA8. Full details can be found on the UniProt database. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human SIRT5 |
| Other Notes: | Synthetic peptide located within the following region: HCDLCLVVGTSSVVYPAAMFAPQVA |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |