Anti-CUGBP2 antibody produced in rabbit
SIGMA/AV40324 - affinity isolated antibody
Synonym: Anti-BRUNOL3; Anti-CUG triplet repeat, RNA binding protein 2; Anti-ETR-3; Anti-NAPOR
Product Type: Chemical
Immunoblotting Cell Type: placenta (Cat. No. AV40324). Lanes 1. Antibody dilution (concentration) 1 μg/mL in cell type placenta
Western Blotting Western Blot of CUGBP2 in Human Jurkat with CUGBP2 antibody at 1 μg/mL
Western Blotting Western Blot of CUGBP2 in Human 721_B with CUGBP2 antibody at 1 μg/mL
Western Blotting Western Blot of CUGBP2 in Human Fetal Heart with CUGBP2 antibody at 1 μg/mL
Western Blotting Western Blot of CUGBP2 in Human Fetal Liver with CUGBP2 antibody at 1 μg/mL
Western Blotting Western Blot of CUGBP2 in Human Fetal Lung with CUGBP2 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 56 kDa |
| NCBI accession no. | NP_006552   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rabbit, mouse, dog, horse, rat, guinea pig, human, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | O95319 |
| Application: | Anti-CUGBP2 polyclonal antibody is used to tag RNA binding protein 2 proteins for detection and quantitation by Western blotting and in cells and tissues by immunohistochemical (IHC) techniques. It is used as a probe to study the role of RNA binding protein 2 in the regulation of posttranslational gene transcription in response to gentotoxic injury. |
| Biochem/physiol Actions: | Anti-CUGBP2 polyclonal antibody reacts with chicken, zebrafish, bovine, human, mouse, rat, and canine RNA binding protein 2 proteins. |
| Biochem/physiol Actions: | Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CUGBP2 (ETR-3/NAPOR/BRUNOL3) is an RNA-binding protein, posttranscriptional controller of gene expression, that regulates key cellular responses such as apoptosis by binding to AU-rich sequences in the mRNA of important genes such as COX-2 and VEGF. CUGBP2 is a critical regulator of the apoptotic response to genotoxic injury in breast cancer cells and mitotic catastrophe response. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human CELF2 |
| Other Notes: | Synthetic peptide located within the following region: NIKTLPGMHHPIQMKPADSEKSNAV |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |