Anti-ACO1 antibody produced in rabbit
SIGMA/AV40390 - affinity isolated antibody
Synonym: Anti-Aconitase 1, soluble
Product Type: Chemical
Immunohistochemistry Anti-ACO1: Cat. No. AV40390: Immunohistochemistry of ACO1 in Human Muscle tissue with ACO1 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: fetal kidney (Cat. No. AV40390). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type fetal kidney
Immunoblotting ACO1: Cat. No. AV40390: Western blot analysis of ACO1 in HePG2 cell lysates with ACO1 antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 98 kDa |
| NCBI accession no. | NP_002188   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | pig, rat, human, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P28271 |
| Application: | Anti-Aconitase-1 antibody is a rabbit IgG polyclonal antibody used to tag Aconitase-1 protein(s) for detection and quantitation by Western blotting and in cells and tissues by immunohistochemical (IHC) techniques. |
| Biochem/physiol Actions: | ACO1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5′ UTR of ferritin mRNA, and in the 3′ UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. |
| Biochem/physiol Actions: | Anti-Aconitase-1 antibody recognizes human aconitase-1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Aconitase-1 is a soluble/cytoplasmin, non-mitochondrial enzyme that catalyzes the stereo-specific isomerization of citrate to isocitrate. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human ACO1 |
| Other Notes: | Synthetic peptide located within the following region: MSNPFAHLAEPLDPVQPGKKFFNLN |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |