Anti-XPO1 antibody produced in rabbit
SIGMA/AV40465 - affinity isolated antibody
Synonym: Anti-CRM1; Anti-DKFZp686B1823; Anti-Exportin 1 (CRM1 homolog, yeast)
Product Type: Chemical
Immunohistochemistry XPO1 Antibody: Cat. No. AV40465: Immunohistochemistry analysis of XPO1 in Human Pineal Tissue with XPO1 Antibody 5.0 μg/mL. Observed Staining: Cytoplasmic in pinealocytes
Immunoblotting Cell Type: fetal heart (Cat. No. AV40465). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type fetal heart
Western Blotting Western Blot of XPO1 in Human HepG2 with XPO1 antibody at 1 μg/mL
Western Blotting Western Blot of XPO1 in Human HeLa with XPO1 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 123 kDa |
| NCBI accession no. | NP_003391   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, pig, horse, rabbit, human, dog, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | O14980 |
| Application: | Anti-XPO1 polyclonal antibody is used to tag exportin 1 (CRM1 homolog, yeast) for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of exportin 1 management of cell function via control of the localization of regulatory proteins, splicing proteins and enzymes. |
| Biochem/physiol Actions: | Anti-XPO1 polyclonal antibody reacts with bovine, canine, human, mouse, and rat exportin 1 (CRM1 homolog, yeast) proteins. |
| Biochem/physiol Actions: | XPO1 mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. XPO1 also regulates NFAT and AP-1.The protein encoded by this gene mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Exportin 1 (CRM1 homolog, yeast) (XPO1, CRM1) is a nuclear export protein that mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1controls cell function by regulating the localization of factors such as Rev, U snRNAs, kinetoplastid spliced leader RNA, cyclin B, MPAK, MAPKAP kinase2 and hexokinase 2 (Hxk2). |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human XPO1 |
| Other Notes: | Synthetic peptide located within the following region: NKLGGHITAEIPQIFDAVFECTLNM |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |