Anti-EIF2S1 antibody produced in rabbit
SIGMA/AV40508 - IgG fraction of antiserum
Synonym: Anti-EIF-2; Anti-EIF-2A; Anti-EIF-2alpha; Anti-EIF2; Anti-EIF2A; Anti-Eukaryotic translation initiation factor 2, subunit 1 α, 35 kDa
Product Type: Chemical
Immunohistochemistry Anti-EIF2S1: Cat. No. AV40508: Immunohistochemistry of EIF2S1 in Human Kidney tissue with EIF2S1 antibody at 4-8 μg/mL.
Immunohistochemistry Anti-EIF2S1: Cat. No. AV40508: Immunohistochemistry of EIF2S1 in Human Kidney tissue with EIF2S1 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV40508). Lanes 1. Antibody dilution (concentration) 1.25 μg/mL in cell type HepG2
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 35 kDa |
| NCBI accession no. | NP_004085   |
| shipped in | wet ice |
| species reactivity | bovine, human, goat, guinea pig, rat, mouse, horse, dog, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P05198 |
| Biochem/physiol Actions: | The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD), and gamma (52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha.The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987).[supplied by OMIM]. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human EIF2S1 |
| Other Notes: | Synthetic peptide located within the following region: RGVFNVQMEPKVVTDTDETELARQM |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |