Anti-PRPF6 (AB2) antibody produced in rabbit
SIGMA/AV40735 - IgG fraction of antiserum
Synonym: Anti-ANT-1; Anti-PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae); Anti-TOM
Product Type: Chemical
Immunoblotting Cell Type: transfected 293T (Cat. No. AV40735). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type transfected 293T
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 104 kDa |
| NCBI accession no. | NP_036601   |
| shipped in | wet ice |
| species reactivity | dog, guinea pig, human, bovine, rabbit, mouse, rat, horse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | O94906 |
| Application: | Anti-PRPF6 (AB2) polyclonal antibody is used to tag PRP6 pre-mRNA processing factor 6 homolog for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of PRP6 pre-mRNA processing factor 6 homolog in spliceosome assembly and autosomal-dominant retinitis pigmentosa. |
| Biochem/physiol Actions: | Anti-PRPF6 (AB2) polyclonal antibody reacts with bovine, human, mouse, rat, zebrafish, and canine PRP6 pre-mRNA processing factor 6 proteins. |
| Biochem/physiol Actions: | PRPF6 appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. PRPF6 also can bind androgen receptor, providing a link between transcriptional activation and splicing.The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6) is involved in pre-mRNA spliceosome assembly by acting as a link between small nuclear ribonucleoproteins (snRNP) U5 snRNP and U4/U6 snRNP to form U4/U6-U5 tri-snPNP. Mutation of PRPF6 are linked to impairment of pre-mRNA splicing and autosomal-dominant retinitis pigmentosa. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human PRPF6 |
| Other Notes: | Synthetic peptide located within the following region: PGKRTVGDQMKKNQAADDDDEDLND |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |