Anti-DND1 antibody produced in rabbit
SIGMA/AV41198 - affinity isolated antibody
Synonym: Anti-Dead end homolog 1 (zebrafish); Anti-MGC34750; Anti-RBMS4
Product Type: Chemical
Immunohistochemistry DND1 Antibody: Cat. No. AV41198: Immunohistochemistry analysis of DND1 in Human Pineal Tissue with DND1 Antibody 5.0 μg/mL. Observed Staining: Cytoplasmic in pinealocytes
Immunoblotting Cell Type: Jurkat (Cat. No. AV41198). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type Jurkat
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 39 kDa |
| NCBI accession no. | NP_919225   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q8IYX4 |
| Application: | Anti-DND1 polyclonal antibody is used to tag dead end homolog 1 proteins for detection and quantitation by Western blotting and in cells and tissues by immunohistochemical (IHC) techniques. It is used as a probe to study the role of dead end homolog 1 proteins in germ-cell and germ cell-tumor development, especially at the level of miRNA mediated repression of mRNA transcription. |
| Biochem/physiol Actions: | Anti-DND1 polyclonal antibody reacts with human, mouse, rat, bovine, and canine dead end homolog 1 proteins. |
| Biochem/physiol Actions: | DND1 contains 2 RRM (RNA recognition motif) domains. It may play a role during primordial germ cell (PGC) development. However, DND1 does not seem to be essential for PGC migration |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Dead end homolog 1 (zebrafish) is an RNA binding protein found in primordial germ cells during embryogenesis. DND1 is essential for germ cell survival. Dnd1 appears to have a critical role in germ-cell and germ cell-tumor development. Dnd1 acts, at least in part, by protecting certain mRNAs from micro-RNA (miRNA)-mediated repression. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human DND1 |
| Other Notes: | Synthetic peptide located within the following region: HRFWYQVVIPGHPVPFSGLIWVVLT |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |