Anti-GPX3 antibody produced in rabbit
SIGMA/AV41491 - affinity isolated antibody
Synonym: Anti-GPx-P; Anti-GSHPx-3; Anti-GSHPx-P; Anti-Glutathione Peroxidase 3 (plasma)
Product Type: Chemical
Immunohistochemistry GPX3 Antibody: Cat. No. AV41491: Immunohistochemistry analysis of GPX3 in Human Lung Tissue with GPX3 Antibody 5.0 μg/mL. Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells
Immunoblotting Cell Type: HepG2 (Cat. No. AV41491). Lanes 1. Antibody dilution (concentration) 1.0 μg/mL in cell type HepG2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 25 kDa |
| NCBI accession no. | NP_002075   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P22352 |
| Application: | Anti-GPX3 polyclonal antibody is used to tag glutathione peroxidase-3 protein for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe to study the antioxidant lipid protecting role of glutathione peroxidase 3 in extracellular space and plasma. |
| Biochem/physiol Actions: | Anti-GPX3 polyclonal antibody reacts with human, mouse, rat, bovine, canine, and pig glutathione peroxidase 3 proteins. |
| Biochem/physiol Actions: | GPX3 belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination.Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme. GPX3 expression appears to be tissue-specific. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glutathione peroxidase refers to a family of isozymes that protect organisms from oxidative damage by reducing lipid hydroperoxides to their corresponding alcohols. Glutathione peroxidase 3 (GPX3) is an extracellular Gpx isozyme found mainly in plasma. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human GPX3 |
| Other Notes: | Synthetic peptide located within the following region: DCHGGISGTIYEYGALTIDGEEYIP |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |