Anti-OTC antibody produced in rabbit
SIGMA/AV41766 - IgG fraction of antiserum
Synonym: Anti-OCTD; Anti-Ornithine carbamoyltransferase
Product Type: Chemical
Immunohistochemistry Anti-OTC: Cat. No. AV41766: Immunohistochemistry of OTC in Human Intestine tissue with OTC antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV41766). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type HepG2
Western Blotting Western Blot of OTC in Human HepG2 with OTC antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 39 kDa |
| NCBI accession no. | NP_000522   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | pig, bovine, horse, sheep, mouse, rat, goat, human, dog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P00480 |
| Application: | Anti-OTC polyclonal antibody is used to tag ornithine transcarbamylase for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of ornithine transcarbamylase in the urea cycle. |
| Biochem/physiol Actions: | Anti-OTC polyclonal antibody reacts with canine, human, mouse, rat, bovine, and pig ornithine carbamoyltransferase/orni |
| Biochem/physiol Actions: | OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ornithine carbamoyltransferase/orni |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human OTC |
| Other Notes: | Synthetic peptide located within the following region: AFRNGHNFMVRNFRCGQPLQNKVQL |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |