Anti-SQLE antibody produced in rabbit
SIGMA/AV42101 - affinity isolated antibody
Synonym: Anti-FLJ30795; Anti-Squalene epoxidase
Product Type: Chemical
Immunohistochemistry Anti-SQLE: Cat. No. AV42101: Immunohistochemistry of SQLE in Human Liver tissue with SQLE antibody at 4-8 μg/mL.
Immunohistochemistry SQLE Antibody: Cat. No. AV42101: Immunohistochemistry analysis of SQLE in Human Brain with SQLE Antibody Antibody Concentration: 4.0-8.0 μg/mL. Cellular Data: Epithelial cells of renal tubule
Immunoblotting Cell Type: 721_B_lymphoblasts (Cat. No. AV42101). Lanes 1. Antibody dilution (concentration) 0.25 μg/mL in cell type 721_B_lymphoblasts
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 39 kDa |
| NCBI accession no. | NP_003120   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, bovine, rabbit, mouse, sheep |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q14534 |
| Application: | Anti-SQLE polyclonal antibody is used to tag squalene epoxidase for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of squalene epoxidase in sterol, ergosterol and cholesterol, biosynthesis. |
| Biochem/physiol Actions: | Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Squalene epoxidase (SQLE) is an NADPH-dependent flavoprotein monooxygenase that oxidizes squalene to 2,3,-oxidosqualene (squalene epoxide) which is the first oxygenation and rate-limiting step in sterol, ergosterol and cholesterol, biosynthesis. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human SQLE |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: KKSFYWARKTSHSFVVNILAQALYE |
| Specificity: | Anti-SQLE polyclonal antibody reacts with human, mouse, rat, pig, zebrafish, bovine, and canine squalene epoxidases. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |