Anti-EDG8 (AB2) antibody produced in rabbit
SIGMA/AV42661 - IgG fraction of antiserum
Synonym: Anti-Edg-8; Anti-Endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; Anti-S1P5; Anti-S1PR5; Anti-SPPR-1; Anti-SPPR-2
Product Type: Chemical
Immunohistochemistry Anti-EDG8 (ab2): Cat. No. AV42661: Immunohistochemistry of EDG8 in Human Intestine tissue with EDG8 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: Jurkat (Cat. No. AV42661). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type Jurkat
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 16 kDa |
| NCBI accession no. | NP_110387   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, dog, human, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9H228 |
| Biochem/physiol Actions: | EDG8 is a receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. It Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). It may play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human EDG8 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: MESGLLRPAPVSEVIVLHYNYTGKL |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |