Anti-UBE2D1 antibody produced in rabbit
SIGMA/AV43006 - IgG fraction of antiserum
Synonym: Anti-E2(17)KB1; Anti-SFT; Anti-UBC4/5; Anti-UBCH5; Anti-UBCH5A; Anti-
Product Type: Chemical
Immunoblotting Cell Type: Jurkat (Cat. No. AV43006). Lanes 1. Antibody dilution (concentration) 1.25 μg/mL in cell type Jurkat
Western Blotting Western Blot of UBE2D1 in recombinant protein with UBE2D1 antibody at 1 μg/mL
Western Blotting Western Blot of UBE2D1 in Rat Skeletal Muscle with UBE2D1 antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 16 kDa |
| NCBI accession no. | NP_003329   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | horse, bovine, pig, goat, rat, dog, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | P51668 |
| Biochem/physiol Actions: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the middle region of human UBE2D1 |
| Other Notes: | Synthetic peptide located within the following region: SQWSPALTVSKVLLSICSLLCDPNP |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |