Anti-PRPF19 (AB2) antibody produced in rabbit
SIGMA/AV43158 - IgG fraction of antiserum
Synonym: Anti-NMP200; Anti-PRP19; Anti-PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae); Anti-PSO4; Anti-UBOX4; Anti-hPSO4
Product Type: Chemical
Immunohistochemistry Anti-PRPF19 (ab2): Cat. No. AV43158: Immunohistochemistry of PRPF19 in Human Kidney tissue with PRPF19 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: Jurkat (Cat. No. AV43158). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type Jurkat
Western Blotting Western Blot of PRPF19 in mouse brain with PRPF19 antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 55 kDa |
| NCBI accession no. | NP_055317   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | guinea pig, mouse, dog, rabbit, rat, human, horse, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9UMS4 |
| Biochem/physiol Actions: | PRPF19 plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. It binds double-stranded DNA in a sequence-nonspecific manner. PRPF19 acts as a structural component of the nuclear framework. It may also serve as a support for spliceosome binding and activity. It is essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. It also may have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.In S. cerevisiae, Pso4 has pleiotropic functions in DNA recombination and in error-prone nonhomologous end-joining DNA repair.[supplied by OMIM]. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the middle region of human PRPF19 |
| Other Notes: | Synthetic peptide located within the following region: PSVVGAGEPMDLGELVGMTPEIIQK |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |