Anti-GOT2 (AB2) antibody produced in rabbit
SIGMA/AV43518 - IgG fraction of antiserum
Synonym: Got2 Antibody; Got2 Antibody - Anti-GOT2 (AB2) antibody produced in rabbit; Anti-FLJ40994; Anti-
Product Type: Chemical
Immunohistochemistry Anti-GOT2 (ab2): Cat. No. AV43518: Immunohistochemistry of GOT2 in Human Liver tissue with GOT2 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV43518). Lanes 1. Antibody dilution (concentration) 1.25 μg/mL in cell type HepG2
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 45 kDa |
| NCBI accession no. | NP_002071   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, rabbit, dog, mouse, horse, human, guinea pig, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P00505 |
| Application: | Anti-GOT2 (AB2) polyclonal antibody is used to tag mitochondrial aspartate aminotransferase protein for detection and quantitation by Western blotting and in cells and tissues by immunohistochemical (IHC) techniques. It is used as a probe to study the role of mitochondrial aspartate aminotransferase in energy metabolism and amino acid composition management by cells. |
| Biochem/physiol Actions: | Anti-GOT2 (AB2) polyclonal antibody reacts with bovine, pig, chicken, human, mouse, rat, and zebrafish mitochondrial aspartate aminotransferases. |
| Biochem/physiol Actions: | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.Glutamic-oxaloac |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glutamic-oxaloacetic transaminase 2 (GOT2) in an inner-membrane mitochondrial enzyme that interconverts the amino acids aspartate and glutamate and the TCA cycle components α-ketoglutarate and oxaloacetate. The enzyme facilitates a balance between energy metabolism and amino acid composition. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human GOT2 |
| Other Notes: | Synthetic peptide located within the following region: AILNTPDLRKQWLQEVKVMADRIIG |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |