Anti-C8B (AB2) antibody produced in rabbit
SIGMA/AV44211 - affinity isolated antibody
Synonym: Anti-Complement component 8, β polypeptide; Anti-MGC163447
Product Type: Chemical
Immunohistochemistry Anti-C8B (ab2): Cat. No. AV44211: Immunohistochemistry of C8B in Liver tissue with C8B antibody at 5.0 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV44211). Lanes 1. Antibody dilution (concentration) 0.25 μg/mL in cell type HepG2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 20 kDa |
| NCBI accession no. | NP_000057   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, dog, mouse, bovine |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P07358 |
| Application: | Anti-C8B (AB2) antibody produced in rabbit is suitable for western blotting at a concentration of 0.25μg/ml and for immunohistochemistry of paraffin-embedded tissue sections at a concentration of 4-8μg/ml. |
| Biochem/physiol Actions: | C8 beta is one of the three subunits that comprise the component 8 (C8) of the complement system. C8 participates in the formation of Membrane Attack Complex that results in the lysis of cells. Patients with C8B deficiency are prone to bacteria infection. |
| Biochem/physiol Actions: | C8B (C82) is the beta component that makes up the complement component 8 (C8) protein. The other two components are the alpha and the gamma subunits. C8 is one of the components of membrane attack complex that mediates cell lysis and enables the membrane penetration by the complex. C2B mediates the interaction of C8 and C5b-7 components of the membrane attack complex. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human C8B |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: SGSTTTTRRSCSKTVTKTVIGPDGH |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |