Anti-GUSB antibody produced in rabbit
SIGMA/AV44234 - IgG fraction of antiserum
Synonym: Anti-Glucuronidase, β; Anti-MPS7
Product Type: Chemical
Immunohistochemistry Anti-GUSB: Cat. No. AV44234: Immunohistochemistry of GUSB in Human Lung tissue with GUSB antibody at 4-8 μg/mL.
Immunohistochemistry GUSB Antibody: Cat. No. AV44234: Immunohistochemistry analysis of GUSB in Human Lung with GUSB Antibody Antibody Concentration: 4.0-8.0 μg/mL. Cellular Data: Epithelial cells of renal tubule
Immunoblotting Cell Type: HepG2 (Cat. No. AV44234). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type HepG2
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 72 kDa |
| NCBI accession no. | NP_000172   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, horse, guinea pig, rabbit, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P08236 |
| Application: | Anti-GUSB polyclonal antibody is used to tag β-glucuronidase protein for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe detect the presence of β-glucuronidase as a gene expression normalization reference protein. |
| Biochem/physiol Actions: | GUSB plays an important role in the degradation of dermatan and keratan sulfates. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-Glucuronidase (GUSB), a lysosomal enzyme, catalyzes the breakdown of complex carbohydrates by hydrolyzing β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides. GUSB is a stably expressed gene product that may be used to normalize the expression of other genes in processes such as mesenchymal stem cell differentiation. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human GUSB |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: VLGNKKGIFTRQRQPKSAAFLLRER |
| Specificity: | Anti-GUSB polyclonal antibody reacts with canine, bovine, zebrafish, chicken, human, mouse, rat, and pig β-glucuronidase proteins. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |