Anti-RHOT1 antibody produced in rabbit
SIGMA/AV44817 - affinity isolated antibody
Synonym: Anti-ARHT1; Anti-FLJ11040; Anti-FLJ12633; Anti-MIRO-1; Anti-Ras homolog gene family, member T1
Product Type: Chemical
Immunohistochemistry Anti-RHOT1: Cat. No. AV44817: Immunohistochemistry of RHOT1 in Human Muscle tissue with RHOT1 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV44817). Lanes 1. Antibody dilution (concentration) 0.25 μg/mL in cell type HepG2
Immunoblotting RHOT1 Antibody: Cat. No. AV44817: Western Blot analysis of RHOT1 in Human Muscle cell lysates with RHOT1 Antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| form | buffered aqueous solution |
| mol wt | 71 kDa |
| NCBI accession no. | NP_060777   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rabbit, horse, bovine, rat, human, mouse, guinea pig, dog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q8IXI2 |
| Application: | Anti-RHOT1 polyclonal antibody is suitable for use in western blotting and immunohistochemical (IHC) techniques. The antibody is used as a probe to determine the role of RHOT1 in calcium-sensitive mitochondrial trafficking. |
| Biochem/physiol Actions: | Mitochondrial GTPase involved in mitochondrial trafficking. RHOT1 is probably involved in control of anterograde transport of mitochondria and their subcellular distribution. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-RHOT1 polyclonal antibody reacts with RHOT1 in chicken, bovine, human, zebrafish, pig, rat, canine, and mouse. |
| General description: | Ras homolog gene family, member T1 (RHOT1, MIRO-1) is an atypical Rho GTPase involved in calcium sensitive mitochondrial trafficking. Miro interacts with the kinesin-binding proteins GRIF-1 and OIP106 forming a link between the mitochondria and the trafficking apparatus of the microtubules. Miro1 and the kinesin adaptor Grif-1 play an important role in regulating mitochondrial transport in neurons. Miro1 is a calcium sensor for glutamate receptor-dependent localization of mitochondria at synapses. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human RHOT1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: MKKDVRILLVGEPRVGKTSLIMSLV |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |