Anti-CRELD1 antibody produced in rabbit
SIGMA/AV45004 - IgG fraction of antiserum
Synonym: Anti-AVSD2; Anti-CIRRIN; Anti-Cysteine-rich with EGF-like domains 1; Anti-DKFZP566D213
Product Type: Chemical
Immunohistochemistry Anti-CRELD1: Cat. No. AV45004: Immunohistochemistry of CRELD1 in Human Kidney tissue with CRELD1 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV45004). Lanes 1. Antibody dilution (concentration) 5.0 μg/mL in cell type HepG2
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 45 kDa |
| NCBI accession no. | NP_001070883   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | dog, guinea pig, bovine, horse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q96HD1 |
| Application: | Anti-CRELD1 polyclonal antibody is used to tag cysteine-rich with EGF-like domains 1 proteins for detection and quantitation by Western blotting and in cells and tissues by immunohistochemical (IHC) techniques. It is used as a probe to study the role of cysteine-rich with EGF-like domains 1 in cardiac diseases such as AVSD and CDH. |
| Biochem/physiol Actions: | Anti-CRELD1 polyclonal antibody detects canine, human, bovine, mouse, and and rat cysteine-rich with EGF-like domains 1 proteins. |
| Biochem/physiol Actions: | Epidermal growth factor (EGF; MIM 131530)-like repeats are a class of cysteine-rich domains that mediate interactions between proteins of diverse function. EGF domains are found in proteins that are either completely secreted or have transmembrane regions that tether the protein to the cell surface. CRELD1 is the founding member of a family of matricellular proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cysteine-rich with EGF-like domains 1 (CRELD1), a highly conserved membrane bound extracellular protein that defines a new epidermal growth factor-related gene family, is a cell adhesion molecule associated with cardiac atrioventricular septal defects (AVSD) and congenital heart disease (CHD). |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human CRELD1 |
| Other Notes: | Synthetic peptide located within the following region: TEVCPGENKQCENTEGGYRCICAEG |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |