Anti-NR3C2 antibody produced in rabbit
SIGMA/AV45599 - IgG fraction of antiserum
Synonym: Anti-MCR; Anti-MGC133092; Anti-MLR; Anti-MR; Anti-Nuclear receptor subfamily 3, group C, member 2
Product Type: Chemical
Immunohistochemistry Anti-NR3C2: Cat. No. AV45599: Immunohistochemistry of NR3C2 in Human Kidney tissue with NR3C2 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: A172 (Cat. No. AV45599). Lanes 1. Antibody dilution (concentration) 1.25 μg/mL in cell type A172
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 107 kDa |
| NCBI accession no. | NP_000892   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P08235 |
| Application: | Anti-NR3C2 polyclonal antibody is used to tag mineralocorticoid receptor protein for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of mineralocorticoid receptor in the mineralocorticoid and glucocorticoid ligand-dependent control of salt balance and water retention. |
| Biochem/physiol Actions: | Anti-NR3C2 polyclonal antibody reacts with chicken, human, mouse, and rat mineralocorticoid/ aldosterone receptors. |
| Biochem/physiol Actions: | NR3C2 is a receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. It binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mineralocorticoid receptor/nuclear receptor subfamily 3, group C, member 2/ (NR3C2, MLR, MCR) is a nuclear receptor for mineralocorticoids (eg. aldosterone) and glucocorticoids (eg. cortisol) that activates expression of proteins involved with ionic (salt balance) and water transport such as the epithelial sodium channel, Na+/K+ pump and serum and glucocorticoid induced kinase (SGK1). |
| Immunogen: | Synthetic peptide directed towards the middle region of human NR3C2 |
| Other Notes: | Synthetic peptide located within the following region: RRKNCPACRLQKCLQAGMNLGARKS |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |