Anti-CDT1 antibody produced in rabbit
SIGMA/AV46350 - affinity isolated antibody
Synonym: Anti-Chromatin licensing and DNA replication factor 1; Anti-DUP; Anti-RIS2
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of CDT1 in Mouse Brain with CDT1 antibody at 4-8 μg/mL
Immunoblotting Cell Type: HepG2 (Cat. No. AV46350). Lanes 1. Antibody dilution (concentration) 1.0 μg/mL in cell type HepG2
Western Blotting Western Blot of CDT1 in Rat brain with CDT1 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 60 kDa |
| NCBI accession no. | NP_112190   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, pig, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9H211 |
| Application: | Anti-CDT1 antibody produced in rabbit is suitable for western blotting at a concentration of 1μg/ml. |
| Biochem/physiol Actions: | CDT1 (chromatin licensing and DNA replication factor 1) gene also referred to as DUP or RIS2 encodes for a protein that is a nuclear localizing replication initiation factor and is expressed only during the G1 and S phases of the cell cycle. CDT1 interacts with CDC6 and stimulates the loading of the mini-chromosome maintenance complex onto chromatin. Hence it forms a pre-replication complex necessary to initiate DNA replication. Further, geminin inhibits CDT1 and may facilitate the inhibition of replication at inappropriate origins. Overexpression of Cdt1 mRNA and geminin may play a crucial role in pathogenesis of acute leukemia (AL). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human CDT1 |
| Other Notes: | Synthetic peptide located within the following region: PATPPATPPAASPSALKGVSQDLLE |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |