Anti-SMC3 antibody produced in rabbit
SIGMA/AV47590 - affinity isolated antibody
Synonym: Anti-BAM; Anti-BMH; Anti-CDLS3; Anti-CSPG6; Anti-HCAP; Anti-SMC3L1; Anti-Structural maintenance of chromosomes 3
Product Type: Chemical
Immunohistochemistry Anti-SMC3: Cat. No. AV47590: Immunohistochemistry of SMC3 in Testis tissue with SMC3 antibody at 5.0 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV47590). Lanes 1. Antibody dilution (concentration) 0.25 μg/mL in cell type HepG2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 74 kDa |
| NCBI accession no. | NP_005436   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | horse, bovine, rat, human, guinea pig, dog, mouse, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9UQE7 |
| Application: | Rabbit Anti-SMC3 antibody is suitable for western blot applications at a concentration of 0.25μg/ml. The product can also be used for IHC applications. |
| Biochem/physiol Actions: | SMC3 belongs to the SMC3 subfamily of SMC proteins. SMC3 occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SMC3 codes for a part of the cohesion complex that regulates chromosome segregation during mitosis. It is known to form a heterodimer with SMC1, which subsequently can restructure the double helical form of DNA into a series of loops. Studies have reported that the opening of the Smc3-Scc1 gate is needed for removal of cohesion from chromosomes during prophase. However, in Drosophila, the disengagement of Smc3/kleisin interface releases cohesin from chromosomes during mitosis and interphase. Rabbit Anti-SMC3 antibody recognizes human, mouse, rat, chicken, zebrafish, bovine, and canine SMC3. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human SMC3 |
| Other Notes: | Synthetic peptide located within the following region: GKATLVMKKGDVEGSQSQDEGEGSG |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |