Anti-PGK1 (AB1) antibody produced in rabbit
SIGMA/AV48140 - affinity isolated antibody
Synonym: Anti-MGC117307; Anti-MGC142128; Anti-MGC8947; Anti-MIG10; Anti-PGKA; Anti-
Product Type: Chemical
Immunoblotting Cell Type: HeLa (Cat. No. AV48140). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type HeLa
Immunoblotting PGK1: Cat. No. AV48140: Western blot analysis of PGK1 in Human Fetal Brain cell lysates with PGK1 antibody at 1.0 μg/mL.
Western Blotting Western Blot of PGK1 in Human Fetal Brain with PGK1 antibody at 1 μg/mL
Western Blotting Western Blot of PGK1 in Mouse Kidney with PGK1 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 44 kDa |
| NCBI accession no. | NP_000282   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | horse, sheep, bovine, rat, human, guinea pig, dog, mouse, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | P00558 |
| Application: | Rabbit Anti-PGK1 antibody has been used as a loading control in immunoblotting assays. The product is suitable at a concentration of 0.5μg/ml for western blots. |
| Biochem/physiol Actions: | PGK1 is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The protein may also act as a cofactor for polymerase alpha.The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. A pseudogene of this gene has been found on the X-chromosome and another on chromosome 19. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. A pseudogene of this gene has been found on the X-chromosome and another on chromosome 19. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Phosphoglycerate kinase 1 (PGK1) is an enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis. PGK1 expression has been correlated to CXCR4 expression, survival and bone marrow metastasis in neuroblastoma patients. Rabbit Anti-PGK1 antibody recognizes bovine, human, mouse, rat, and canine PGK1. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human PGK1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: PEVEKACANPAAGSVILLENLRFHV |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |