Anti-P4HB (AB2) antibody produced in rabbit
SIGMA/AV48151 - affinity isolated antibody
Synonym: Anti-DSI; Anti-ERBA2L; Anti-GIT; Anti-PDI; Anti-PDIA1; Anti-PHDB; Anti-PO4DB; Anti-
Product Type: Chemical
Immunohistochemistry P4HB Antibody: Cat. No. AV48151: Immunohistochemistry analysis of P4HB in Human Liver Tissue with P4HB Antibody 5.0 μg/mL. Observed Staining: Cytoplasm in hepatocytes
Immunohistochemistry Immunohistochemistry of P4HB in Human Lung Tissue with P4HB antibody at 4-8 μg/mL
Immunoblotting Cell Type: HepG2 (Cat. No. AV48151). Lanes 1. Antibody dilution (concentration) 0.25 μg/mL in cell type HepG2
Western Blotting Western Blot of P4HB in Mouse Small Intestine with P4HB antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 55 kDa |
| NCBI accession no. | NP_000909   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | horse, pig, human, dog, rabbit, rat, bovine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P07237 |
| Application: | Rabbit Anti-P4HB antibody has been used for western blot applications at a dilution of 1:1500. |
| Biochem/physiol Actions: | P4HB is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Prolyl 4-hydroxylase, β polypeptide (P4HB) is a disulphide isomerase that catalyzes the creation, cleavage and arrangement of disulfide linkages. It is known to function as a chaperone during protein folding activities. Studies have reported that P3HB-b-P4HB block copolymers have a better yield and tensile strength. Rabbit Anti-P4HB antibody recognizes bovine, chicken, rabbit, human, mouse, and rat P4HB. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human P4HB |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: DRTVIDYNGERTLDGFKKFLESGGQ |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |