Anti-ATP5B (AB2) antibody produced in rabbit
SIGMA/AV48186 - IgG fraction of antiserum
Synonym: Anti-ATP synthase, H+ transporting, mitochondrial F1 complex, β polypeptide; Anti-ATPMB; Anti-ATPSB; Anti-MGC5231
Product Type: Chemical
Immunohistochemistry Anti-ATP5B (ab2): Cat. No. AV48186: Immunohistochemistry of ATP5B in tissue with ATP5B antibody at 4-8 μg/mL.
Immunohistochemistry Immunohistochemistry of ATP5B in Human Brain with ATP5B antibody at 4-8 μg/mL
Immunoblotting Cell Type: HepG2 (Cat. No. AV48186). Lanes 1. Antibody dilution (concentration) 1.25 μg/mL in cell type HepG2
Western Blotting Western Blot of ATP5B in Human NT-2, mouse, rat brain with ATP5B antibody at 1 μg/mL
Western Blotting Western Blot of ATP5B in Human Fetal Liver, Human Fetal Lung, Human Ovary Tumor, Human 786-0 with ATP5B antibody at 1 μg/mL
Western Blotting Western Blot of ATP5B in Mouse Skeletal Muscle with ATP5B antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 52 kDa |
| NCBI accession no. | NP_001677   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, bovine, dog, guinea pig, mouse, rabbit, horse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | P06576 |
| Biochem/physiol Actions: | ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B is the beta subunit of the catalytic core.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human ATP5B |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: MGKLVPLKETIKGFQQILAGEYDHL |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |