Anti-ENO3 (AB1) antibody produced in rabbit
SIGMA/AV48204 - affinity isolated antibody
Synonym: Anti-Enolase 3 (β, muscle); Anti-MSE
Product Type: Chemical
Immunohistochemistry Anti-ENO3 (ab1): Cat. No. AV48204: Immunohistochemistry of ENO3 in tissue with ENO3 antibody at 4-8 μg/mL.
Immunohistochemistry ENO3 Antibody: Cat. No. AV48204: Immunohistochemistry analysis of ENO3 in heart with ENO3 Antibody 5.0 μg/mL. Observed Staining: Cytoplasmic
Immunoblotting Cell Type: Jurkat (Cat. No. AV48204). Lanes 1. Antibody dilution (concentration) 0.25 μg/mL in cell type Jurkat
Immunoblotting ENO3 Antibody: Cat. No. AV48204: Western Blot analysis of ENO3 in Human heart cell lysates with ENO3 Antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 47 kDa |
| NCBI accession no. | NP_001967   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P13929 |
| Application: | Rabbit Anti-ENO3 antibody is suitable for western blot applications at a concentration of 0.25μg/ml. |
| Biochem/physiol Actions: | ENO3 is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5′ UTR. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Enolase 3 (ENO3) is a skeletal muscle isoenzyme that is involved in muscle development. ENO3 transcription is regulated by muscle-specific enhancer proteins. Mutations in ENO3 have been linked to glycogen storage diseases. Rabbit Anti-ENO3 antibody recognizes bovine, canine, rabbit, human, mouse, and rat ENO3. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human ENO3 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: EVDLHTAKGRFRAAVPSGASTGIYE |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |