Anti-TNP1 antibody produced in rabbit
SIGMA/AV53607 - affinity isolated antibody
Synonym: Anti-TP1; Anti-Transition protein 1 (duRing Histone to protamine replacement)
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of TNP1 in Human Testis with TNP1 antibody at 4-8 μg/mL
Immunoblotting Cell Type: 721_B (Cat. No. AV53607). Lanes 1. Antibody dilution (concentration) 1 μg/mL in cell type 721_B
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 6 kDa |
| NCBI accession no. | NP_003275   |
| shipped in | wet ice |
| species reactivity | mouse, dog, human, rat, rabbit, horse, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | P09430 |
| Application: | Anti-TNP1 antibody produced in rabbit is suitable for western blotting at a concentration of 1μg/mL. |
| Biochem/physiol Actions: | In mammals, the second stage of spermatogenesis is characterised by the conversion of nucleosomal chromatin to compact, non-nucleosomal and transcriptionally inactive form found in sperm nucleus. This condensation is associated with a double-protein transition. The first transition corresponds to the replacement of histones by several spermatid-specific proteins (also called transition proteins) which are themselves replaced by protamines during the second transition. In the elongating spermatids of mammals, the conversion of nucleosomal chromatin to the compact, non-nucleosomal form found in the sperm nucleus is associated with the appearance of a small set of basic chromosomal transition proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | TNP1 (transition protein 1) gene also referred to as TP1 encodes a 54 amino acids containing nuclear protein that belongs to nuclear transition protein 1 family. It is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. It is also a basic protein well conserved in mammalian species. It has a role in spermiogenesis. Mutation in TNP1 gene leads to male infertility. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human TNP1 |
| Other Notes: | Synthetic peptide located within the following region: MSTSRKLKSHGMRRSKSRSPHKGVK |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |