Anti-GCLC antibody produced in rabbit
SIGMA/AV54576 - affinity isolated antibody
Synonym: Anti-GCS; Anti-GLCL; Anti-GLCLC; Anti-
Product Type: Chemical
Immunohistochemistry GCLC Antibody: Cat. No. AV54576: Immunohistochemistry analysis of GCLC in Human Bronchial Epithelial Tissue with GCLC Antibody 1.0 μg/mL. Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue
Immunoblotting Cell Type: fetal liver (Cat. No. AV54576). Lanes 1. Antibody dilution (concentration) 1 μg/mL in cell type fetal liver
Immunoblotting GCLC Antibody: Cat. No. AV54576: Western Blot analysis of GCLC in Human Liver cell lysates with GCLC Antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 73 kDa |
| NCBI accession no. | NP_001489   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, dog, rat, mouse, bovine, guinea pig, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P48506 |
| Application: | Anti-GCLC antibody produced in rabbit is suitable for western blotting at a concentration of 1μg/mL. |
| Biochem/physiol Actions: | GCLC gene encodes the heavy catalytic subunit of the enzyme glutamate-cysteine ligase, which is the rate limiting enzyme of glutathione synthesis. Mutation at this locus is associated with hemolytic anemia due to deficiency of γ-glutamylcysteine synthetase. Polymorphism in GCLC subunit is found to be associated with sulfamethoxazole-induced hypersensitivity in HIV/AIDS patients in a study. A GAG-trinucleotide repeat polymorphism in the 5′-untranslated region of the gene is shown to lower the levles of GCL activity and GSH (glutathione), a major intracellular antioxidant. Therefore, it is associated with increased risk for lung and aerodigestive tract cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human GCLC |
| Other Notes: | Synthetic peptide located within the following region: VLETLQEKGERTNPNHPTLWRPEYG |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |