Bacteriorhodopsin from Halobacterium salinarum
SIGMA/B0184 - native sequence, lyophilized powder
Synonym: BR from H. salinarum; Bacterioopsin; Bacteriorhodopsin from Halobacterium halobium
CAS Number: 53026-44-1
MDL Number: MFCD00130599
Product Type: Chemical
| biological source | Halobacterium salinarium |
| form | lyophilized powder |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | ligand binding assay: suitable |
| mass spectrometry (MS): suitable | |
| UniProt accession no. | B0R5N9  |
| Application: | Bacteriorhodopsin from Halobacterium salinarum has been used: • in generation of droplet lipid bilayer • as a standard in quadrupole time-of-flight (QTOF) mass spectroscopy (MS) • in the generation of protein-detergent complex and micelles for dynamic light scattering studies |
| Application: | Bacteriorhodopsin is of interest in the development of artificial retinas, optical associative processors, and three-dimensional memory storage devices. |
| Biochem/physiol Actions: | A transmembrane retinylidine protein that functions as a proton pump driven by light energy in Holobacterium. |
| Biochem/physiol Actions: | Bacteriorhodopsin (BR) from Halobacterium salinarum acts as a proton-driven pump. BR can be used in studies of the folding and kinetics of α-helical proteins. It is thermally stable and exhibits high photoelectric and photochemical efficiency. BR exists as trimer in a hexagonal lattice. Its photocycle intermediates are exploited in bioelectronics majorly in photoelectric and photochemical applications. |
| General description: | Bacteriorhodopsin (BR) is a covalent complex comprising bacterioopsin protein and retinal cofactor in the equimolar ratio. It corresponds to the molecular weight of 27kDa. BR belongs to the retinylidene class of proteins. It is a seven-membrane helical protein that acts as a photon-driven pump. BR can be used in studies of the folding and kenetics of β-helical proteins. |
| General description: | Bacteriorhodopsin is the prototypical "seven-helix" transmembrane protein (with seven α-helical domains), whose study led to advances in understanding G protein-coupled receptors (GPCRs). In Halobacteria, it acts as a light-harvesting protein, producing a proton gradient across the cell wall that is then used to drive biosynthetic processes. |
| Preparation Note: | Aqueous suspensions may be sonicated to achieve the desired homogeneity and may be stored for a short time at a temperature of 2-8 °C or at a temperature of -20 °C without time limitation. |
| Preparation Note: | Wild-type bacteriorhodopsin is isolated from Halobacterium salinarum strain S9 as purple membranes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |