Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse
SIGMA/B0271 - clone GAL-13, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-β-Galactosidase
Product Type: Chemical
ELISA
Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody, Mouse monoclonal, Clone: GAL-13 (Cat. No. B0271), followed by ExtrAvidin®-Peroxidase, was serially diluted to detect β-Galactosidase by indirect ELISA.
Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody, Mouse monoclonal, Clone: GAL-13 (Cat. No. B0271), followed by ExtrAvidin®-Peroxidase, was serially diluted to detect β-Galactosidase by indirect ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GAL-13, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bacteria |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: 1:2,000 |
| Application: | Monoclonal Anti-β-Galactosidase-Biot |
| Biochem/physiol Actions: | β-galactosidase enzyme is encoded by lacZ gene of E. coli; it acts on lactose and cleaves it to glucose and galactose. Monoclonal Anti-β-Galactosidase-Biot |
| Biochem/physiol Actions: | The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-b-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ß-Galactosidase enzyme is encoded by lacZ gene of E. coli. |
| Immunogen: | β-galactosidase from E. coli |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| Preparation Note: | Prepared by conjugation with ε-aminocaproyl biotin. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |