5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

SIGMA/B0274 - tablet

Synonym: BCIP^® p-toluidine salt; X-phosphate p-toluidine salt

CAS Number: 6578-06-9
Empirical Formula (Hill Notation): C₈H₆BrClNO₄P · C₇H₉N
Molecular Weight: 433.62
EC Number: 229-506-1
MDL Number: MFCD00040636
Linear Formula: C₈H₆BrClNO₄P · C₇H₉N
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B0274-10TAB	10 tabs	$412.00 1/EA	Add To Favorites
45-B0274-25TAB	25 tabs	$819.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

form	tablet
InChI	1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key	QEIFSLUFHRCVQL-UHFFFAOYSA-N
Quality Level	200
SMILES string	Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
storage temp.	−20°C

Application:	For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Application:	For the colorimetric detection of alkaline phosphatase-labeled molecules.
Biochem/physiol Actions:	Substrate of choice for use with alkaline phosphatase in immunoblotting and, less commonly, in immunohistological staining procedures. High assay sensitivity is achieved via amplification when BCIP is used in conjunction with Nitro Blue Tetrazolium (NBT) Tablets (Catalog No. N5514). This substrate produces an insoluble end product that is blue-purple in color and can be observed visually.
General description:	Histochemical substrate for alkaline phosphatase.
Legal Information:	BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging:	10, 25 tabs in glass bottle
Preparation Note:	Contains 25 mg substrate per tablet.

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352204

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