Monoclonal Anti-FITC−Biotin antibody produced in mouse
SIGMA/B0287 - clone FL-D6, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-FITC
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:1,000 Monoclonal Anti-Actin, α-Smooth Muscle -FITC antibody produced in Mouse, Clone: 1A4 (Cat. No. A2547) followed by 1:400 Monoclonal Anti-FITC -Biotin antibody produced in Mouse, Clone: FL-D6 (Cat. No. B0287) and 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FL-D6, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil |
| Application: | Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used: • for amplification of signal in immunofluorescence assays • in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5 • enzyme linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin. |
| Biochem/physiol Actions: | The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | FITC-BSA conjugate |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |