Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat
SIGMA/B0529 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat anti-Mouse IgG
MDL Number: MFCD00162621
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded section of human appendix stained with Monoclonal Anti-Actin, α-Smooth Musclet (Cat. No. A2547) followed by 1:300 Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B0529) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human placenta stained with 20 μg/mL Monoclonal Anti-Cytokeratin, pan antibody produced in Mouse clone PCK-26 (Cat. No. C5992) followed by Anti-Mouse IgG (Fab specific-Biotin antibody produced in goat (Cat. No. B0529) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Human plasma were separated on SDS-PAGE and probed with Anti-Factor VII antibody, Mouse monoclonal, Clone: HVII-1 (Cat. No. F8146). The antibody was developed with 4 μg/mL Anti-Mouse IgG (Fab specific)-Biotin (Cat. No. B0529) followed by 1 μg/mL ExtrAvidin®-Alkaline Phosphatase (Cat. No. E2636). Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:200,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300 | |
| western blot: 1:300,000-1:600,000 using using Hela cells extract |
| Application: | Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat has been used in immunohistochemistry and immunofluorescence studies. |
| Application: | Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat is suitable for direct ELISA at a dilution of 1:200000 and for immunohistochemistry at a dilution of 1:300. The antibody was used to stain paraffin-embedded, rat skin, duodenum, esophagus and spleen sections at a dilution of 1:200. For immunofluorescence of Human Gingival Fibroblasts, a dilution of 1:200 was used. For immunoblotting experiments using mouse adipose tissue, antibody dilution of 1:30000 was used. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Binds all mouse Igs. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. |
| Other Notes: | Antibody adsorbed with human IgG and rat serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with human or rat samples. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |