Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)−Biotin Conjugate antibody, Mouse monoclonal
SIGMA/B0555 - clone 260.4, KDR-2, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 antibody produced in mouse; Anti-KDR; Anti-VEGFR-2
Product Type: Chemical
Immunoblotting Human VEGF Receptor-2 (KDR) fusion protein (Cat. No. V6758) was separated on SDS-PAGE and probed with 1:1,000 Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)?Biotin Conjugate, Clone: 260.4, KDR-2 (Cat. No. B0555). The antibody was developed using ExtrAvidin®-Peroxidase (Cat. No. E2886) and a chemiluminescent substrate. Lanes 1. 0.1 μg VEGF Receptor-2 (KDR) fusion protein 2. 0.2 μg VEGF Receptor-2 (KDR) fusion protein
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 260.4, KDR-2, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:1,000 using insect cell culture supernatant expressing human extracellular recombinant VEGF R |
| UniProt accession no. | P35968  |
| Application: | Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)-Biotin Conjugate antibody produced in mouse has also been used in : • enzyme linked immunosorbent assay (ELISA) • immunoblotting • flow cytometry • immunohistochemistry • immunohistochemistry |
| Biochem/physiol Actions: | Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)-Biotin Conjugate antibody is purified from ascites fluid, and conjugated withSulfo-NHS-LC-Biotin. It is specific for human VEGF receptor2. It does not cross react with Flt1, Flt4 or platelet-derived growth factor receptor β (PDGF-R1). |
| Biochem/physiol Actions: | Reacts specifically with human VEGF receptor-2. This clone has also been termed 260.4. It does not recognize Flt1 (VEGFR-1), Flt4 (VEGFR-3), or platelet-derived growth factor receptor (PDGF-R). |
| Biochem/physiol Actions: | Vascular endothelial growth factors receptors (VEGF Rs) are receptor tyrosine kinases that bind to VEGF that plays important role in embryonic vasculogenesis, angiogenesis and homeostasis. VEGF R2 has the ability to form heterodimers with VEGF R1 and VEGF R3. The binding of VEGF to VEGF R2 results in activation of tyrosine amino acids of the cytoplasmic tail of the receptor. This interaction results in the activation of MAPK, PI3K, PKC, FAK and Src kinase pathways. The biological roles of VEGF R2 include in epithelial cell growth, proliferation, survival, migration and vascular permeability and angiogenesis. Due to its role in promoting migration and angiogenesis, VEGF R2 has been implicated in breast and prostate cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Biotin Conjugated Monoclonal Anti-VEGF Receptor-2 (KDR) (mouse IgG1 isotype) is derived from the KDR-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified recombinant human extracellular VEGF receptor-2 (KDR). Vascular endothelial growth factor (VEGF) is also called vasculotropin (VAS), vascular permeability factor (VPF) and kinase-insert domain containing receptor (KDR8). It is a member of a family of endothelial cell mitogens and angiogenic factors. VEGF is a homodimeric heparin-binding glycoprotein. The KDR gene has been mapped to human chromosome 4q11-q12. The homologous gene for the human KDR gene is mouse Flk1 (fetal liver kinase 1) and rat TKrc. |
| General description: | The KDR receptor (VEGFRa), defining marker of hematopoietic stem cells (HSCs), distinguishes pluripotent HSCs from lineage-committed progenitor HSCs. |
| Immunogen: | purified recombinant human extracellular VEGF receptor-2 (KDR). |
| Physical form: | Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |