Anti-B23 antibody, Mouse monoclonal
SIGMA/B0556 - clone FC82291, purified from hybridoma cell culture
Synonym: Anti-NPM; Anti-Nucleophosmin
MDL Number: MFCD03457543
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500 Monoclonal Anti-B23 Clone: FC82291 (Cat. No. B0556). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting
3T3 Mouse cell extract was separated on SDS-PAGE and probed with Monoclonal Anti-B23 (Nucleophosmin/NPM), clone FC82291(Cat. No. B0556). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase (Cat. No. A2179) and an NBT/BCIP substrate.
Antibody dilution 0.2 μg/mL.
3T3 Mouse cell extract was separated on SDS-PAGE and probed with Monoclonal Anti-B23 (Nucleophosmin/NPM), clone FC82291(Cat. No. B0556). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase (Cat. No. A2179) and an NBT/BCIP substrate.
Antibody dilution 0.2 μg/mL.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-B23 Clone: FC82291 (Cat. No. B0556). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FC82291, monoclonal |
| concentration | ~0.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 37 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, kangaroo rat, canine, bovine, human, mouse, monkey, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable using 2% formaldehyde-acetone or 10% formalin/methanol-1% NP-40 |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 0.2-0.4 μg/mL using a whole extract of 3T3 (mouse fibroblasts) cells | |
| UniProt accession no. | P06748  |
| Application: | The antibody may be used for ELISA, competitive ELISA, immunoblotting (37 kDa), immunoprecipitation, immunocytochemistry (2% formaldehyde-acetone 1,2,5 or 10% formalin/methanol-1% NP-40 and microinjection (blocks the initiation of centrosome duplication) and immunohistochemistry. Reactivity has been observed with human, monkey, bovine, dog, hamster (weak), rat,kangaroo rat, and mouse B23. |
| Biochem/physiol Actions: | It detects both the phosphorylated and the unphosphorylated B23 molecule. The epitope recognized by the antibody lies within the 68 amino acids at the C-terminus of B23. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase A activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E. Phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor a (RARa). As a consequence, a fusion protein (60-7 0 kDa) is produced containing parts of the two genes. |
| Immunogen: | rat B23 (nucleophosmin, NPM, numatrin). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| Preparation Note: | Purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |