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5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt

SIGMA/B0631 - ≥90%

Synonym: 5-BrdUTP

CAS Number: 102212-99-7
Empirical Formula (Hill Notation): C9H14BrN2O14P3
Molecular Weight: 547.04
MDL Number: MFCD00057405
Linear Formula: C9H14BrN2O14P3
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-B0631-1MG 1 mg
$150.00
1/EA
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45-B0631-5MG 5 mg
$491.00
1/EA
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45-B0631-25MG 25 mg
$1970.00
1/EA
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assay ≥90%
biological source synthetic (organic)
form powder
InChI 1S/C9H14BrN2O14P3.Na.H/c10-4-2-12(9(15)11-8(4)14)7-1-5(13)6(24-7)3-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h2,5-7,13H,1,3H2,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI key UWZGQOWZUSBZMC-UHFFFAOYSA-N
Quality Level 200 
SMILES string [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=C(Br)C(=O)NC2=O
solubility water: 50 mg/mL, clear, colorless to faintly yellow
storage temp. −20°C
Application: 5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.
Application: 5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:
• in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cells
• to label cleaved DNA ends in apoptosis assay in avian tissue sections
• for incorporation into mice prostates for immunohistochemistry studies

General description: 5-Bromo-2′-deoxyuridine 5′-triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles.
Packaging: 1, 5 mg in glass bottle
Packaging: 25 mg in poly bottle
Purity ≥90%
Storage Temp. −20°C
UNSPSC 41106305

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