Anti-BACE 1, N-Terminus (46-62) antibody produced in rabbit
SIGMA/B0681 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-β-Site APP Cleaving Enzyme
MDL Number: MFCD03454775
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-BACE 1, N-Terminus (46-62) antibody produced in Rabbit (Cat. No. B0681) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing BACE 1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat cerebellum sections with 20 μg/mL Rabbit Anti-BACE-1, N-Terminus (46-62) (Cat. No. B0681) using biotin/ExtrAvidin®-Peroxidase.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 60-75 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:1,000 using a whole cell extract from the human kidney HEK293 cell line stably transfected with human BACE-1 |
| UniProt accession no. | P56817  |
| Application: | Anti-BACE 1, N-Terminus (46-62) antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Overexpression of β-site APP cleaving enzyme, Asp2 or memapsin 2 (BACE-1) leads to increased β-secretase activity while displaying appropriate cleavage site specificity for APP. The N-glycosylation and phosphorylation of BACE-1 within its C-terminal domain regulated its intracellular trafficking. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The membrane-associated aspartic protease BACE-1 (β-site APP cleaving enzyme, Asp2 or memapsin 2) has been identified as β-secretase. BACE-1 constitutes the predominant β-secretase activity in human brain tissue. It is highly expressed in neurons, the major site of Aβ generation. BACE-1 is localized within the Golgi and endosomal compartments, among the several intracellular sites where Aβ is thought to be produced. BACE-1 is initially an inactive proenzyme and localized in endoplasmic reticulum. |
| Immunogen: | synthetic peptide corresponding to the N-terminus of human BACE-1 (amino acids 46-62). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |