Anti-BACE 1, C-Terminus (485-501) antibody produced in rabbit
SIGMA/B0806 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-β-Site APP Cleaving Enzyme
MDL Number: MFCD03454775
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-BACE 1, C-Terminus (485-501) antibody produced in Rabbit (Cat. No. B0806) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing BACE 1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat cerebellum sections with 20 μg/mL Rabbit Anti-BACE 1, C-Terminus (485-501) (Cat. No. B0806) using biotin/ExtrAvidin®-Peroxidase.
Western Blotting Whole extract of HEK-293T cells expressing BACE 1 were separated on SDS-PAGE and probed with Anti-BACE 1, C-Terminus (485-501) antibody produced in Rabbit (Cat. No. B0806). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. 1:1,000 antibody + inhibition with immunizing peptide 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 70 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:1,000 using a whole cell extract from the human kidney HEK293 cell line stably transfected with human BACE-1 | |
| UniProt accession no. | P56817  |
| Application: | Anti-BACE 1, C-Terminus (485-501) antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Overexpression of β-site APP cleaving enzyme (BACE-1) leads to increased β-secretase activity while displaying appropriate cleavage site specificity for amyloid precursor protein (APP). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | BACE-1 (β-site APP cleaving enzyme, Asp2 or memapsin 2) is known as β-secretase. BACE-1 is highly expressed in neurons, the major site of Aβ generation. BACE-1 is localized within the Golgi and endosomal compartments, among the several intracellular sites where Aβ is thought to be produced. It constitutes the predominant β-secretase activity in human brain tissue. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of human BACE-1 (amino acids 485-501). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |