BAFF active human
SIGMA/B0939 - Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
Synonym: B lymphocyte stimulator; B-cell-activating factor BAFF; BLyS; Dendritic cell-derived TNF-like molecule; TALL-1; TNF- and APOL-related leukocyte expressed ligand 1
Product Type: Chemical
Immunoblotting BAFF active humanCat. No. H0939: SDS-PAGE analysis of recombinant BAFF. Samples were loaded in 15% SDS polyacrylamide gel and stained with Coomassie blue. Lanes 1. Molecular weight marker (kDa) 2. 300 ng of rhuman BAFF 3. Analysis of recombinant BAFF with specific anti-human BAFF by WB; contains 300 ng of rhuman BAFF. All bands shown in lanes 2-3 have been identified by MALDI-TOFF as recombinant BAFF.
| assay | >97% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | Endotoxin, tested (LAL test, < 0.04 EU/ ug protein) |
| mol wt | 18-20 kDa (a glycosilated polypeptide chain containing 151 amino acids) |
| packaging | pkg of 10 μg |
| potency | ≤50 ng/mL ED50 |
| Quality Level | 200  |
| recombinant | expressed in Nicotiana |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | Q9Y275  |
| Analysis Note: | The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. ED50 ≤ 50 ng/mL |
| General description: | BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B |
| Other Notes: | Extinction Coefficient: E0.1% = 0.791 (A280 nm) p.I: 6.03 |
| Physical form: | Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl. |
| Preparation Note: | Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |