Anti-BACH1 antibody produced in rabbit
SIGMA/B1310 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-
MDL Number: MFCD06201924
Product Type: Chemical
Immunofluorescence MCF7 Human breast carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 20 μg/mL Anti-BACH1 antibody produced in Rabbit (Cat. No. B1310) followed by 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Picture C: cells were counterstained with DAPI (blue) to stain nuclei (Picture A: without DAPI, Picture B: only DAPI).
Immunoblotting MCF7 Human breast carcinoma cells were separated on SDS-PAGE and probed with 1:2,000 Rabbit Anti-BACH1 (Cat. No. B1310). The antibody was developed with HRP conjugated Anti-Rabbit IgG antibody and ECL substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~150 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using RIPA extract (0.5 mg) of human HeLa cells |
| indirect immunofluorescence: 10-20 μg/mL using human MCF-7 cells | |
| microarray: suitable | |
| western blot: 0.5-1.0 μg/mL using whole extracts of MCF-7 human breast carcinoma cells | |
| UniProt accession no. | Q9BX63  |
| Application: | Anti-BACH1 antibody produced in rabbit has been used in: • immunoblotting • immunohistochemistry • western blotting |
| Application: | Polyclonal anti-BACH1 antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10 μg by using RIPA extract (0.5 mg) of human HeLa cells and for indirect immunofluorescence at 10-20 μg/mL using human MCF-7 cells. The product is also suitable for western blot analysis at a working concentration of 0.5-1.0 μg/mL by using whole extracts of MCF-7 human breast carcinoma cells. Further, anti-BACH1 antibody has shown suitability for microarray analysis. |
| Biochem/physiol Actions: | BACH1 interacts directly with the C-terminal BCRT repeats in Breast cancer type1 protein (BRCA1), a tumor suppressor nuclear phosphoprotein that facilitates the repair of double-stranded DNA breaks. Bach1 also functions as transcription repressor in transfection assays using fibroblast cells and as a transcriptional activator and repressor, respectively, in cultured erythroid cells. Additionally, it is also involved in the coordination of transcription activation and repression by Maf family members such as MafK. Bach1 (BTB and CNC homology 1, basic leucine zipper transcription factor 1) inhibits oxidative stress-inducible genes and is a negative regulator of oxidative stress-induced cellular senescence. |
| Biochem/physiol Actions: | BTB Domain And CNC Homolog 1 (BACH1) interaction between breast cancer type 1 (BRCA1) in vivo appears to be dependent on phosphorylation of Ser990 in the latter during the G2/M phase of the cell cycle. BACH1 mutations were found in patients with early-onset breast cancer. It displays a punctate nuclear staining pattern similar to that observed for BRCA1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | BACH1, BRCA1 interacting protein C-terminal helicase 1, encodes a protein that is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand br |
| General description: | BTB Domain And CNC Homolog 1 (BACH1) is expressed in numerous tissues. The protein includes a nuclear localization signal in its helical domain. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 1233-1249 of human BACH1 with N-terminal added cysteine, conjugated to KLH. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |