Monoclonal Anti-Phosphotyrosine−Biotin antibody produced in mouse
SIGMA/B1531 - clone PT-66, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-Phosphotyrosine; Phospho-Tyr; Phospho-tyrosine; p-Tyr
Product Type: Chemical
ELISA Monoclonal Anti-Phosphotyrosine-Biotin antibody produced in Mouse, Clone: PT-66 (Cat. No. B1531), followed by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), was serially diluted to detect Phosphotyrosine by direct ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PT-66, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000 |
| dot blot: 1:32,000 |
| Application: | Monoclonal Anti-Phosphotyrosine?Biot |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP. |
| General description: | Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine?biot |
| Immunogen: | phosphotyrosine conjugated to BSA |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |