Bone Morphogenetic Protein 2 human
SIGMA/B1814 - Carrier Free, ≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: Bone Morphogenetic Protein 2 human; BMP-2
MDL Number: MFCD03097877
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Bioassay
EC50 is defined as the effective concentration of
BMP-2 that elicits a 50% attachment factor in cell based bioassay.
Cell line: ACTD-5.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | Endotoxin, tested |
| mol wt | 26 kDa |
| packaging | pkg of 10 μg |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble 0.100 mL, clear, colorless |
| storage condition | avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.) |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P12643  |
| Analysis Note: | The biological activity is measured by its ability to induce alkaline phosphatase production by ATDC5 chondrogenic cells. |
| Biochem/physiol Actions: | Cellular responses to BMP-2 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. BMPs stimulate angiogenesis by inducing the production of VEGF-A by osteoblasts. Human, mouse, and rat BMP-2 demonstrate 100% homology. |
| Physical form: | Lyophilized from a 0.2 μm filtered buffered solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | 104.0 °F - closed cup |
| Flash Point(C) | 40.0 °C - closed cup |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |