Monoclonal Anti-BrdU antibody produced in mouse
SIGMA/B2531 - clone BU-33, ascites fluid, Immunohistology Grade
Synonym: Anti Brdu; Anti Brdu Antibody - Monoclonal Anti-BrdU antibody produced in mouse; Brdu Antibody; Anti-
MDL Number: MFCD00164550
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: TIG3 tumor suppressor-dependent organelle redistribution and apoptosis in skin cancer cells. By: Scharadin, T. M., Jiang, H., et al. in PLoS One, 2011. PubMed ID: 21858038 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0023230), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
Proliferating cells in the eye of E4 chick embryo were stained with Monoclonal Anti-BrdU, clone no. BU-33 (Cat. No. B2531) and developed using Rabbit Anti-Mouse IgG (whole molecule)-FITC.
From R.S. Goldstein, Dept. of Life Science, Bar-Ilan University, Ramat Gan, Israel.
Proliferating cells in the eye of E4 chick embryo were stained with Monoclonal Anti-BrdU, clone no. BU-33 (Cat. No. B2531) and developed using Rabbit Anti-Mouse IgG (whole molecule)-FITC.
From R.S. Goldstein, Dept. of Life Science, Bar-Ilan University, Ramat Gan, Israel.
Immunohistochemistry
Triple sections from E4 chick embryo: Nervous system (pink) is stained with Monoclonal Anti-HNK-1/N-CAM, clone no. VC1.1 (Cat. No. C0678) and Goat Anti-Mouse IgG-Peroxidase (Cat. No. A3673). Nuclei that incorporated BrdU are stained with Monoclonal Anti-BrdU, clone no. BU-33 (Cat. No. B2531) and Goat Anti-Mouse IgM-Alkaline Phosphatae (Cat. No. A7784). BrdU-negative nuclei were stained with Harris′ hematoxylin (Cat. No. H5522). From Dr. R.S. Goldstein, Dept. of Life Sciences, Bar-Ilan university, Ramat Gan, Israel.
Triple sections from E4 chick embryo: Nervous system (pink) is stained with Monoclonal Anti-HNK-1/N-CAM, clone no. VC1.1 (Cat. No. C0678) and Goat Anti-Mouse IgG-Peroxidase (Cat. No. A3673). Nuclei that incorporated BrdU are stained with Monoclonal Anti-BrdU, clone no. BU-33 (Cat. No. B2531) and Goat Anti-Mouse IgM-Alkaline Phosphatae (Cat. No. A7784). BrdU-negative nuclei were stained with Harris′ hematoxylin (Cat. No. H5522). From Dr. R.S. Goldstein, Dept. of Life Sciences, Bar-Ilan university, Ramat Gan, Israel.
Hetrokaryons between proliferating (BrdU pre-labeled) and quiescent (unlabeled) endothelial cells were treated with TNF-α to induce programmed cell death (apoptosis). BrdU incorporation into DNA was detected with Monoclonal Anti-BrdU, (clone no. BU-33, Cat. No. B2531) followed by goat Anti-Mouse IgG-β-galactosidase. After staining with acridine orange (Cat. No. A6014) cells were visualized by phase contrast (left) and fluorescent (right) microscopy. Tailed arrows indicate intact (solid) or apoptotic (dotted) BrdU positive nuclei, arrow heads point to normal (solid) and apoptotic (notched) BrdU negative nuclei. From V. Polunovsky, Univ. of Minnesota, Minneapolis, MN.
Immunohistochemistry
Neural tube (spinal cord) of E4 chick embryo stained with Monoclonal Anti-BrdU (clone no. BU-33, Cat. No. B2531) and hematoxylin.
From Dr. R.S. Goldstein, Dept. of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Neural tube (spinal cord) of E4 chick embryo stained with Monoclonal Anti-BrdU (clone no. BU-33, Cat. No. B2531) and hematoxylin.
From Dr. R.S. Goldstein, Dept. of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Staining of BrdU from 4th embryonic day (E4) chicks using Monoclonal Anti-BrdU (clone no. BU-33, Cat. No. B2531). Spinal cord. From Dr. R. Goldstein, Bar-Ilan University, Ramat Gan, Israel.
Staining of BrdU from 4th embryonic day (E4) chicks using Monoclonal Anti-BrdU (clone no. BU-33, Cat. No. B2531). Eye. From Dr. R. Goldstein, Bar-Ilan University, Ramat Gan, Israel.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BU-33, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using sections of intestine from mouse or rat treated in vivo with BrdU |
| Application: | Monoclonal Anti-BrdU antibody has been used in immunostaining, BrdU incorporation into DNA, immunohistochemistry and uterine labeling index. It has also been used to investigate cell proliferation. |
| Biochem/physiol Actions: | 5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo. |
| Biochem/physiol Actions: | Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | 5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Monoclonal anti-BrdU (immunohistology grade) (mouse IgG1 isotype) is derived from the BU-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with bromodeoxyuridine conjugated to KLH. |
| Immunogen: | bromodeoxyuridine conjugated to KLH. |
| Other Notes: | This product can be found as purified product that was produced using cell culture hybridoma product. B8434 Anti-BrdU antibody, Mouse monoclonal clone BU-33, purified from hybridoma cell culture |
| Preparation Note: | For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |