Anti-fd Bacteriophage−Biotin antibody produced in rabbit
SIGMA/B2661 - ~3.5 mg/mL, IgG fraction of antiserum
Synonym: Anti-fd Bacteriophage
Product Type: Chemical
ELISA Anti-fd Bacteriophage-Biotin antibody produced in Rabbit (Cat. No. B2661), followed by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), was serially diluted to detect fd Bacteriophage by indirect ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~3.5 mg/mL |
| conjugate | biotin conjugate |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells |
| Application: | Anti-fd Bacteriophage-Biotin antibody produced in rabbit was used to access immunoglobulin content using bead-based immunoassays. |
| Biochem/physiol Actions: | The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | fd bacteriophage |
| Physical form: | Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |