Bone Morphogenetic Protein 4 human
SIGMA/B2680 - >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Synonym: Bone Morphogenetic Protein 4 human; BMP-4
MDL Number: MFCD01867024
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
| assay | >95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <0.01 EU/μg Endotoxin level (LAL test) |
| mol wt | ~22 kDa by SDS-PAGE |
| monomer calculated mol wt 13 kDa | |
| packaging | pkg of 10 μg |
| potency | 0.70-7.00 ng/mL ED50 |
| Quality Level | 200  |
| recombinant | expressed in NSO cells |
| storage condition | avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.) |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P12644  |
| Analysis Note: | The biological activity is measured by its ability to induce alkaline phosphatase production by C2C12 myoblasts. |
| Biochem/physiol Actions: | Cellular responses to BMP-4 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. BMPs stimulate angiogenesis by inducing the production of VEGF A by osteoblasts. Mature human and mouse BMP-4 demonstrates 98% and 100% homology, respectively, to mature rat BMP-4. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg of bovine serum albumin per 1 μg of cytokine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |